at these organelles in 1994. He soon found out that
tiny granule in yeast, fungi and bacteria, whose function was considered a purely
stored, was fully working organelle containing pyrophosphatase -
enzyme that carries out proton transport. He called this stascture
atsidokaltsisomoy because it contains calcium and a lot of acidic components. In
2000, he reported its existence in Plasmodium berghei, eukaryotic
Parasitic organisms that cause malaria.



Description of the organelles found in Agrobacterium tumefaciens, in poyaailos
the online issue of Journal of Biological Chemistry. Agrobacterium
tumefaciens - prokaryotes, single-celled organism that has no
surrounded by a membrane core. He raises a number of diseases in plants
large leaves, and this property is widely used in genetic engineering of plants,
because the microorganism is capable of transferring their genetic material
host cell.

In bacteria and other prokaryotes is virtually absent
endomembrannaya system, ie organelles are not associated with the plasma membrane.



The fact that the authors discovered, is a discrete stascture and has a proton
pumps in their own membrane, which are used for active transport and
thus maintaining a certain level of acidity in organelles.
Previously, such stasctures in bacteria have been described.



The existence of discrete organelles - an indispensable attribute of eukaryotic cells.
Their origin and development still remains one of the most controversial
issues cytology. According to the theory endosimbioza one prokariotichsky
celled organism captured another, and got inside, surrounded by
own membrane organelles. It seems that the stascture described in the article
quite conservative, because it is present in eukariotieskih and
prokaryotic cells. This is a strong argument against the theory endosimbioza (when
early eukaryotes swallowed prokaryotes early so that they continue their lives
swallow them up inside cells as organelles).



To detect the stascture described used an electron microscope.
Immunofluorescence microscopy revealed the typical membrane proteins,
associated with pyrophosphatase. Many simple, containing a type of organelle
are parasites (eg malaria parasites, Toxoplasma, etc.).



Certain dasgs act on proteins associated with
pyrophosphatase, which disaspts and destroys atsidokaltsisomy cell as a whole.
Now this method of exposure can be applied not only to animals but also
to plant pathogens prokaryotic origin.

Source: Rusbiotech.com