O. Orlov Secret of the prostate taken after vigorous massage of the cancer. Normal amount of fluid varies from 3-4 to 1-2 ml drops. collected it on the fat-free glass slide or n
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Irina Orlova correctness of the analysis is largely determined by the state preanaliticheskogo stages - preparing the patient to the study, taking biological material and its delivery to the laboratory. Spe
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B. S. Tsushko On the basis of physiological function of hemostasis, a new strategy systematic approach to the knowledge of biological and medical phenomena (1,3). In light of these developments, every student of bio
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Malikova SA Despite the fact that the detection of phosphatase activity is one of the long-used enzymatic laboratory tests, he does not lose its significance and now, especially if s hepatobiliary system. This test is always in demand, so every year Laboratory City Hospital № 20 of Krasnoyarsk is held in average about 4000 a year of research to determine the activity of total alkaline phosphatase (ALP), which is about 12% the total amount of all research enzyme activity in seasm. Determination of enzyme activity by a method based on the hydrolysis of p -Npp, the end-point. used a set: 1. ,, Diahim-AP NPF,, Abris + ". (ILA buffer with magnesium sulfate). 2. ,, Novofosfal "CJSC, Vector-Best" (DEA buffer with magnesium chloride). 3. Firms,, Vital Ds "(with glycerine buffer). Inside the laboratory quality control on this method was carried out with using test materials company Medlakor:,, FIDM-control,,, ZHSP control ". At the initial stage of implementation methodology, quality control, as defined in Order № 45 Russian Federation Health Ministry of 07.02. 2000 statistical data processing showed no match results (CV 20%, 20) with the recommended standards (1). work with the literature and analysis of practical results in order to improve the quality of determine the activity of the enzyme, allowed to identify some problems with the definition ALP activity. As a result, we came to the need for compulsory registration conducting this test, the following factors: 1. Standardization of biological material - definition ALP activity should be undertaken only in the seasm. Using anticoagulants leads to a decrease in enzymatic activity. Inhibitory effect of anticoagulants reflected in the chart. 1 activity of seasm alkaline phosphatase. ALP activity of plasma obtained by using as an anticoagulant: 2-heparin, 3-oxalate, citrate, 4, 5-EDTA. greatest inhibitory effect was observed when using EDTA (associated with formation of complexes of EDTA with the ions Mg **) (2). The most acceptable anticoagulant for the determination of ALP activity in plasma is heparin. 2. Standardizing storage time. on this issue in literature there are different opinions. If you can not hold analysis of the day or need to obtain seasm samples for follow-up study next day, we adhere to the following conditions: seasm best stored at room temperature plastic tube with a cork. When freezing activity may decrease, and when stored in the refrigerator - to rise to 10% (3). 3. Features preparation of working solution for the enzymatic reaction , using reagents of different firms. Sets of firms,, Vital Ds ": the buffer and p-Npp fully ready for analysis; do not require further dilution and dilution with distilled water may prepare any minimum amount of working reagent. Indicators control seasm using a working reagent,, Vital Ds " stable until the expiration date. To prepare working reagent in the sets,, Novofosfal-AP "should be used Dist. water free from carbonates. While working solution fit Method for a week, it is better to cook it in the quantity necessary for work for 1-2 days. 4. need production control for each sample especially for sera with high biliasbin levels, otherwise the results will be falsely inflated . Biliasbinmmoll E experience ; units. E control unit. A ~ f (
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Alekhin, SA Lipatov, VA Kursk State Medical University, Dept. Surgical Diseases № 2, dep. operative surgery and topographic anatomy www.drli.h1.com trypsin assay is a
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Alekhin, SA Lipatov, VA Kursk State Medical University, Dept. Surgical Diseases № 2, dep. operative surgery and topographic anatomy www.drli.h1.com Determination of trypsin
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JRS Vladimir Romanov, Ph.D. Petrenko TI diagnosis of hepatitis, parenteral transmission of patients with active TB is very difficult, from
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